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We make no claims regarding the medicinal, preventive or curative properties of wolfberries (lycium barbarum). This product is not intended to diagnose, treat, cure, or prevent disease. The wolfberry fruit has been used in traditional Chinese medicine (TCM) for more than 2000 years. Modern scientists have been researching the potential of wolfberries (lycium barbarum) over the past 20 years. Scroll down to see these research articles posted on the National Institutes of Health (NIH.GOV) website.

 

Lycium Barbarum Polysaccharides Promote Osteoblasts Viability by Regulating microRNA-17/PTEN

2019 May 15 Abstract

Aims: Lycium barbarum polysaccharides (LBPs) have been reported to promote the proliferation of osteoblasts (cells that form new bone). Further, the proliferation of osteoblasts has been proved to be associated with microRNA (miR)-17 which targets phosphatase and tensin homolog (PTEN) 3'-untranslated region (UTR) for regulating phosphoinositide 3 kinase/protein kinase B (PI3K/AKT) signaling pathway. Consequently, we aimed to explore a miR-17-relative mechanism by which LBPs mediates proliferation of osteoblasts.

Materials and methods: LBPs were used to stimulate MC3T3-E1 cells, and SF1670 was applied to repress PTEN. miR-17 inhibitor was transfected into cells for down-regulating miR-17. The cell viability and migration were examined by cell counting kit-8 (CCK-8) and 24-well Transwell migration assay, respectively. The expression of Cyclin D1, matrix metallopeptidase (MMP)-2 and MMP-9 was analyzed using Western blot. Quantitative reverse transcription PCR (qRT-PCR) was conducted to detect miR-17. The expression of PTEN and phosphorylation of PI3K and AKT were analyzed by Western blot.

Key findings: We demonstrated that LBPs promoted osteoblasts viability, facilitated migration and positively regulated the expression of miR-17. Additionally, miR-17 inhibitor abolished the positive effects of LBPs on the osteoblasts. Molecularly, miR-17 inhibited the expression of PTEN by targeting its 3'-untranslated region (3'-UTR). Mechanically, LBPs diminished PTEN expression and facilitated the phosphorylation of PI3K and AKT, while the decreased expression of PTEN and enhanced phosphorylation of PI3K and AKT were reversed by miR-17 inhibitor.

Significance: LBPs might fortify osteoblasts viability by up-regulating miR-17 to target PTEN and consequently trigger PI3K/AKT pathway.

https://pubmed.ncbi.nlm.nih.gov/30951746/

 

*These statements have not been evaluated by the Food and Drug Administration.

This product is not intended to diagnose, treat, cure, or prevent disease.